Application Notes

This experiment shows how the flowbot® ONE was used to automate a PCR set-up prior to amplification on a thermal cycler.
In the program, flowbot ONE produces a master mix and dispense it into a 96 well PCR plate. An identical setup was performed manually to compare the time spent.

This experiment demonstrates the benefits of automating bead clean-up protocols. It documents increased precision and repeatability, reduced error rate, and maximized yield of purified biological materials.

This experiment documents how the flowbot® ONE was optimized for handling dimethyl sulfoxide (DMSO), ethanol, glycerol, and Tween®-20 in different concentrations, and offers specific guidance on which parameters to adjust.

This experiment shows how the flowbot® ONE was used to prepare a dsDNA standard curve for quantitation with Thermo Fisher Scientific’s Quant-iT™ PicoGreen™ dsDNA Reagent.

This experiment shows how the flowbot® ONE was configured with Biotage® ProPlus PhyTip® columns for small scale purification of human IgG samples.

This experiment demonstrates how the flowbot ONE performs dilution and normalization protocols.

The flowbot ONE software features guided templates for both dilution and normalization, making it easy to set up with neither scripting or coding required.

This application note explains how the flowbot® ONE was used to automate qPCR set-up prior to sample processing with a QuantStudio™ 5 Real-Time PCR system.

This work was carried out using a flowbot ONE configured with a 1 channel 2-200uL pipette module and an 8 channel 1-20uL pipette module and featuring an integrated UV light source to limit any contamination between runs.

Discover how the flowbot® ONE automates the isolation of total DNA from tissues, cells, and challenging samples like insects using Macherey-Nagel’s NucleoSpin® 96 DNA RapidLyse kit.

This application note highlights the efficiency and speed of the process, achieving high-quality DNA extraction suitable for various molecular biology applications.

This application note explores the automation of total DNA extraction from various plant samples using Macherey-Nagel’s NucleoSpin® 96 Plant II kit on the flowbot® ONE.

The method ensures high-quality DNA suitable for diverse applications in plant research, including phylogenetics, crop improvement, and genetic engineering.

This application note details the automation of plasmid DNA extraction from bacterial cultures using Macherey-Nagel’s NucleoSpin® 96 Plasmid kit on the flowbot® ONE.

The automated protocol significantly enhances efficiency and reproducibility, providing high-quality plasmid DNA for various research applications, including gene therapy, cloning, and protein production.

This application note demonstrates the automation of DNA extraction from various soil types using Macherey-Nagel’s NucleoSpin® 96 Soil kit on the flowbot® ONE.

The automated process ensures high yield and purity of microbial DNA, crucial for microbiome and metagenomic research, by efficiently eliminating contaminants and inhibitors.

The study shows how automating ELISA for cortisol detection using the flowbot® ONE offers substantial workflow efficiency and data quality improvements, particularly in studies involving multiple participants, such as our cohort of 12 volunteers.