Application Notes

This experiment shows how the flowbot® ONE was used to automate a PCR set-up prior to amplification on a thermal cycler.
In the program, flowbot ONE produces a master mix and dispense it into a 96 well PCR plate. An identical setup was performed manually to compare the time spent.

This experiment demonstrates the benefits of automating bead clean-up protocols. It documents increased precision and repeatability, reduced error rate, and maximized yield of purified biological materials.

This experiment documents how the flowbot® ONE was optimized for handling dimethyl sulfoxide (DMSO), ethanol, glycerol, and Tween®-20 in different concentrations, and offers specific guidance on which parameters to adjust.

This experiment shows how the flowbot® ONE was used to prepare a dsDNA standard curve for quantitation with Thermo Fisher Scientific’s Quant-iT™ PicoGreen™ dsDNA Reagent.

This experiment shows how the flowbot® ONE was configured with Biotage® ProPlus PhyTip® columns for small scale purification of human IgG samples.

This experiment demonstrates how the flowbot ONE performs dilution and normalization protocols.

The flowbot ONE software features guided templates for both dilution and normalization, making it easy to set up with neither scripting or coding required.

This application note explains how the flowbot® ONE was used to automate qPCR set-up prior to sample processing with a QuantStudio™ 5 Real-Time PCR system.

This work was carried out using a flowbot ONE configured with a 1 channel 2-200uL pipette module and an 8 channel 1-20uL pipette module and featuring an integrated UV light source to limit any contamination between runs.