Automate DNA quantitation with PicoGreen™ and the flowbot® ONE

This experiment shows how the flowbot® ONE was used to prepare a dsDNA standard curve for quantitation with Thermo Fisher Scientific’s Quant-iT™ PicoGreen™ dsDNA Reagent.

It was designed around a program created to dispense specified volumes of TE buffer and dsDNA into a 96-well plate before adding a working solution of PicoGreen.

A BioShake 3000-T was integrated into the protocol to ensure thorough mixing.