Protein purification using PhyTip® columns on the flowbot® ONE

This experiment shows how the flowbot® ONE was
configured with Biotage® ProPlus PhyTip® columns for small scale purification of human IgG samples.

A program was created in the flowbot ONE software to capture human IgG from either culture medium or PBS, as control, on ProPlus PhyTip columns prior to washing, elution, and neutralization.

The work was performed in collaboration with YUMAB GmbH.