PCR and qPCR setup

One platform for all your labware

PCR is a very powerful technique that allows the quantification of the amount of DNA or RNA in a given sample. For this reason, reliable liquid handling is of utmost importance to create credible and reproducible results every time.

Flowbot® ONE can handle volumes down to 1 µL.

Automation of your tedious handheld pipetting routines with flowbot® ONE, not only removes the factor of human error, but also free up hands for doing more valuable lab work.

As a user of flowbot® ONE you get an unprecedented large flexibility as you can define the labware that fits your labprocesses, that be tips, vials, tubes or the various PCR, RT-PCR, qPCR setups. All are easily handled by flowbot® ONE.

How do I setup flowbot® ONE for PCR?

With your defined labware on the deck of flowbot® ONE, you are already half way there by virtue of the onboard cameras. Next, the user interface allows a simple and intuitive drag-and-drop function to set up the PCR reactions from scratch or from a master mix.

The user defines pipetting volumes, pipetting orders, and various mixing needs. The choice of a single channel pipette adds the ability to do “cherry picking” whereas an 8 channel pipette generates a system with higher throughput and shorter turn-around cycles. flowbot™ ONE can be equipped to your need.

Automating your PCR setup on flowbot® ONE has several benefits:

Higher reproducibility
Fewer human mistakes

QLife changed their PCR setup from manual to automation with flowbot® ONE

“In the testing facility the robots are used in two main steps: First, the robots help pool patient samples to save time in the downstream analysis as well as reducing the amount of reagents and plastic materials consumed.

In the second step we use flowbots for transferring eluates from patient samples into the PCR plate where it is mixed with enzymatic reagents before the analysis get done on the PCR machine.”

Thomas Danielsen
Assay BiochemistQLife

Would you like to see flowbot® ONE in action?

Get a free demo. You can use the pipette tips of your choice on flowbot® ONE.

PCR is short for Polymerase Chain Reaction (PCR)  and is performed to detect genetic material from a specific organism, such as a virus. A PCR test detects the presence of a virus if you are infected at the time of the test. The test could also detect fragments of virus even after you are no longer infected. Right now we hear a lot about this test due to COVID-19, but it is also used for many other purposes.

qPCR is short for quantitative Polymerase Chain Reaction and is also known as real-time polymerase chain reaction (real-time PCR). It is a laboratory technique that monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time) and thereby not at its end as in conventional PCR.

Real-time PCR can be used quantitatively (quantitative real-time PCR) and semi-quantitatively (i.e., above/below a certain amount of DNA molecules) (semi-quantitative real-time PCR).

PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation. The invention of polymerase chain reaction has proved a revolutionary step for molecular biology. With this extremely powerful technology a short region of DNA can be further amplified with higher order of magnitude to produce thousands to million copies of a specific sequence.