Ready-to-go PCR package for fast, easy and convenient setup
All you need to transfer your reagents, master mixes and samples to your 96 well plate including application support and a protocol template. Here is what’s included:
Includes: 1-channel 1000µl pipette module, 8-channel 200µl pipette module and UV Light
You will get: 200µl pipette tips, 1000µl pipette tips and a pipette tip adapter
The adapter is prepared with a QR code so you can get started right away.
We deliver a template program for your PCR protocol, Training, Installation and Labware recommendations
You need a coldplate and a top adapter. We will prepare it for you with QR codes and the device is already integrated into the flowbot® ONE software.
Racks for primers and reaction buffers are included plus 2 cooling blocks.
Get in touch today!
Fill in the form below and we’ll be in touch with more information about the PCR package:
Automating your PCR setup on flowbot® ONE has several benefits:
In the testing facility the robots are used in two main steps: First, the robots help pool patient samples to save time in the downstream analysis as well as reducing the amount of reagents and plastic materials consumed.
In the second step we use flowbots for transferring eluates from patient samples into the PCR plate where it is mixed with enzymatic reagents before the analysis get done on the PCR machine.
Read the story here
PCR and qPCR on flowbot® ONE
PCR is a very powerful method that detects genetic material from a specific organism and uses repeated temperature cycles to make millions of copies of DNA. Reliable liquid handling is of utmost importance to create credible and reproducible results every time.
With our PCR kit you are ready to carry out configuration of master mixes and sample transfers from 96 well plates right off the bat.
Flowbot® ONE can handle volumes down to 1 µl. Its 1-channel pipette allows you to perform cherry picking while its 8-channel pipette supports a higher throughput and shorter turn-around cycles.
As a user of flowbot® ONE you get an unprecedented amount of flexibility in your PCR, RT-PCR, and qPCR setups, and the intuitive software enables everyone to use it. And, for good measure, you can clean the robot with built-in UV light when you are done.
flowbot® ONE – PCR made easy
PCR is short for Polymerase Chain Reaction (PCR) and is performed to detect genetic material from a specific organism, such as a virus. A PCR test detects the presence of a virus if you are infected at the time of the test. The test could also detect fragments of virus even after you are no longer infected. Right now we hear a lot about this test due to COVID-19, but it is also used for many other purposes.
qPCR is short for quantitative Polymerase Chain Reaction and is also known as real-time polymerase chain reaction (real-time PCR). It is a laboratory technique that monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time) and thereby not at its end as in conventional PCR.
Real-time PCR can be used quantitatively (quantitative real-time PCR) and semi-quantitatively (i.e., above/below a certain amount of DNA molecules) (semi-quantitative real-time PCR).
PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation. The invention of polymerase chain reaction has proved a revolutionary step for molecular biology. With this extremely powerful technology a short region of DNA can be further amplified with higher order of magnitude to produce thousands to million copies of a specific sequence.
More applications for flowbot® ONE
You may often be challenged by having your samples or material in one format, in one orientation or in a container, which isn’t optimal for the down stream processes but to make the transfers into a new format, a new orientation or a different container is just a tedious and time consuming manual job.
Sometimes it makes sense to build your assays in a spread sheet file, which can be manipulated using the standard operations, which you are familiar with. To support this type of modus operandi, all flowbot® ONE steps in any given process can be programmed from a simple CSV format.