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Automating your PCR setup on flowbot® ONE has several benefits:
PCR and qPCR on flowbot® ONE
PCR is a very powerful method that detects genetic material from a specific organism and uses repeated temperature cycles to make millions of copies of DNA. Reliable liquid handling is of utmost importance to create credible and reproducible results every time.
With our PCR kit you are ready to carry out configuration of master mixes and sample transfers from 96 well plates right off the bat.
Flowbot® ONE can handle volumes down to 1 µl. Its 1-channel pipette allows you to perform cherry picking while its 8-channel pipette supports a higher throughput and shorter turn-around cycles.
As a user of flowbot® ONE you get an unprecedented amount of flexibility in your PCR, RT-PCR, and qPCR setups, and the intuitive software enables everyone to use it. And, for good measure, you can clean the robot with built-in UV light when you are done.
flowbot® ONE – PCR made easy
PCR is short for Polymerase Chain Reaction (PCR) and is performed to detect genetic material from a specific organism, such as a virus. A PCR test detects the presence of a virus if you are infected at the time of the test. The test could also detect fragments of virus even after you are no longer infected. Right now we hear a lot about this test due to COVID-19, but it is also used for many other purposes.
qPCR is short for quantitative Polymerase Chain Reaction and is also known as real-time polymerase chain reaction (real-time PCR). It is a laboratory technique that monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time) and thereby not at its end as in conventional PCR.
Real-time PCR can be used quantitatively (quantitative real-time PCR) and semi-quantitatively (i.e., above/below a certain amount of DNA molecules) (semi-quantitative real-time PCR).
PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation. The invention of polymerase chain reaction has proved a revolutionary step for molecular biology. With this extremely powerful technology a short region of DNA can be further amplified with higher order of magnitude to produce thousands to million copies of a specific sequence.