CSV-FILE BASED SETUP
Cherry picking, reorganization, 384-well plate work and more
Sometimes it makes sense to build your assays in a spread sheet file, which can be manipulated using the standard operations, which you are familiar with.
To support this type of modus operandi, all flowbot™ ONE steps in any given process can be programmed from a simple CSV format. It is possible to both save or load your setup files as well as the steps describing the individual pipetting steps of the liquid handling process.
Furthermore, if you want to load concentrations of your material for dilutions protocols or normalization steps, the input can be provided in CSV files. The user interface clearly indicates where you can upload data using CSV files.
What can I use the CSV-files for?
The CSV files allows the experienced user to create quick manipulations of complex protocols. Operating with multiple and long pipetting sequences on 384-well plates can sometimes be quite error-prone. By setting up the complex protocols in CSV files you are able to reduce this risk. CSV files are good to use for:
- Description of your specific deck layout of labware
- Description of individual pipetting steps for both pipetting and dispensing aliquots (save/load)
- Input for normalization
- Input for dilution
- Input for ID tags on individual plates and wells for traceability
When would I use a CSV-file as output?
A flowbot™ONE should make your job easier, this is why we here have listed some of the benefits by using the CSV-file setup:
- Throughout the natural flow of the user interface, options will appear to use CVS files as input files for your setup
- Upload or download templates for your specific changes
- Define a pipetting map of “hits” or “cherries” to be picked and transferred in the CSV files
- Reorganize from plate to plate with source and target ID
- Reformat, e.g., from 96-well microlitre plates to 384-well microlitre plates
- Create macros to automate the CSV files generation
What do I need to get started?
We have made it as easy as possible, so in order for you to get started you need a PC or Mac with spread sheet software, the flowbot™ ONE instruction manual from Flow Robotics and your defined components on your flowbot™ ONE. That’s it – Now you are ready.
More applications for flowbot™ ONE
PCR & qPCR
Flowbot™ ONE offers the accuracy of 1 μL pipetting and with an unprecedented large flexibility for users to define their own labware (tips, vials, tubes), the various PCR, RT-PCR, qPCR setups are easily handled by flowbot™ ONE.
Reformatting, cherry picking, transferring
You may often be challenged by having your samples or material in one format, in one orientation or in a container, which isn’t optimal for the down stream processes but to make the transfers into a new format, a new orientation or a different container is just a tedious and time consuming manual job.