A PCR Workflow on flowbot® ONE
flowbot ONE remains a preferred choice for PCR prep. Here’s why:
Reagent dispensing
The high accuracy of flowbot® ONE ensures consistent and precise volumes across multiple samples, giving correct results and highly reproducible experiments with minimum effort. To keep temperature sensitive reagents safe and cool, flowbot ONE also has an integrated coldplate.
Master mix preparation
Dispensing the master mixes evenly across a PCR 96/384 well plate is optimized with the flowbot® ONE feature ‘contact aspiration’.
Serial dilutions & Normalization
To optimize these processes, the flowbot® ONE carries a pre-programmed template and features like reusing tips, prewetting and mixing to improve the concentration accuracy and template programs.
Sample preparation
Sample preparation on flowbot® ONE is made as secure and accurate as possible with features like automatic liquid level detection, prewetting & mixing and bottom touch.
In the testing facility the robots are used in two main steps: First, the robots help pool patient samples to save time in the downstream analysis as well as reducing the amount of reagents and plastic materials consumed.
In the second step we use flowbots for transferring eluates from patient samples into the PCR plate where it is mixed with enzymatic reagents before the analysis get done on the PCR machine.
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Our ready-to-go PCR package
Our PCR package contains everything you need to get started:
Includes: 1-channel 1000µl pipette module, 8-channel 200µl pipette module and UV Light
You will get: 200µl pipette tips, 1000µl pipette tips and a pipette tip adapter
The adapter is prepared with a QR code so you can get started right away.
We deliver a template program for your PCR protocol, Training, Installation and Labware recommendations
You need a coldplate and a top adapter. We will prepare it for you with QR codes and the device is already integrated into the flowbot® ONE software.
Racks for primers and reaction buffers are included plus 2 cooling blocks.
Benefits of automating your PCR setup with flowbot® ONE
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More applications for flowbot® ONE
Would you like to see flowbot® ONE in action?
Get a free demo. You can use the pipette tips of your choice on flowbot® ONE.